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Structured Review

Real Time Primers real-time primers
Real Time Primers, supplied by Real Time Primers, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/real-time+primers/real+time+primers/pm40323306-190-5-0
Average 90 stars, based on 1 article reviews
real-time primers - by Bioz Stars, 2026-10
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Related Articles

Expressing:

Article Title: miR-146a is Critical for Orchestrating Mycobacterium fortuitum Survival Through Anti-inflammatory and M2 Macrophage Responses in Fish.
Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Article Title: Combined analysis of mRNA and miRNA reveals the mechanism of pacific white shrimp ( Litopenaeus vannamei ) under acute alkalinity stress
Article Snippet: The total RNA used for the previous RNA-Seq and small RNA analysis was reverse transcribed into cDNA using a mixture of Stem loop primers and real-time primers respectively. qRT-PCR cycles were as follows: 30 s at 95°C, followed by 40 cycles of 5 s at 95°C and 30 s at 60°C. β -actin was used as internal controls to normalize the experimental data of the miRNAs and mRNAs.

Article Title: Application of Pulsed Electric Field During Malting: Impact on Fusarium Species Growth and Mycotoxin Production.
Article Snippet: Real-time primers and probes for the quantification of Fusarium species DNA are provided in Table 5.

Article Title: Knockdown of orthotospovirus-derived silencing suppressor gene by plant-mediated RNAi approach induces viral resistance in tomato
Article Snippet: RNA interference (RNAi) shows significant effectiveness in conferring resistance to viral infections in various plants.. The current study was conducted to develop transgenic tomato (Solanum lycopersicum cv.. Rio Grande) lines expressing a 320 bp conserved inverted region of the integral 35S promoter region.

Article Title: Cellular prion: a novel regulator of decidual cell function at the maternal-fetal interface.
Article Snippet: Cellular prion (PRNP) is a GPI-anchored extrinsic membrane glycoprotein, which has been implicated in mouse decidualization.. However, the molecular function of this protein in mouse decidua is not known.. In this article, we have characterized and elucidated the possible role of PRNP in mouse decidua.

Article Title: What Happens Inside the Germinating Grain After Microbial Decontamination by Pulsed Electric Field? Data-Driven Multi-Omics Helps Find the Answer
Article Snippet: Real-time primers and probes for the quantification of Fusarium species DNA are provided in .

Article Title: Selection and validation of reference genes for quantitative expression analysis of regeneration-related genes in Cheilomenes sexmaculata by real-time qRT-PCR.
Article Snippet: The regeneration of missing body parts is a fascinating phenomenon that has captured the interest of scientists since time immemorial.. Several organisms ranging from planaria and echinoderms to vertebrates have the potential to regenerate their distal limb structures or primordia that are lost due to an injury or any experimental manipulation [1, 2].. Recent studies in ladybird beetles (Coleoptera: Coccinellidae) have shown high incidences of regeneration [3, 4].

Article Title: Evaluation of physical, chemical, and microbiological characteristics of waste stabilization ponds, Giza, Egypt
Article Snippet: The outlet parameters of the Atfih WWTP met the Egyptian legislation (the Egyptian Code 501/2015, category D) for the irrigation of trees (Table 2). qPCR specificity, sensitivity, and detection limits Each set of real-time primers and probes utilized in this investigation was assessed for specificity.

Infection:

Article Title: miR-146a is Critical for Orchestrating Mycobacterium fortuitum Survival Through Anti-inflammatory and M2 Macrophage Responses in Fish.
Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Article Title: Combined analysis of mRNA and miRNA reveals the mechanism of pacific white shrimp ( Litopenaeus vannamei ) under acute alkalinity stress
Article Snippet: The total RNA used for the previous RNA-Seq and small RNA analysis was reverse transcribed into cDNA using a mixture of Stem loop primers and real-time primers respectively. qRT-PCR cycles were as follows: 30 s at 95°C, followed by 40 cycles of 5 s at 95°C and 30 s at 60°C. β -actin was used as internal controls to normalize the experimental data of the miRNAs and mRNAs.

Article Title: Application of Pulsed Electric Field During Malting: Impact on Fusarium Species Growth and Mycotoxin Production.
Article Snippet: Real-time primers and probes for the quantification of Fusarium species DNA are provided in Table 5.

Article Title: Knockdown of orthotospovirus-derived silencing suppressor gene by plant-mediated RNAi approach induces viral resistance in tomato
Article Snippet: RNA interference (RNAi) shows significant effectiveness in conferring resistance to viral infections in various plants.. The current study was conducted to develop transgenic tomato (Solanum lycopersicum cv.. Rio Grande) lines expressing a 320 bp conserved inverted region of the integral 35S promoter region.

Article Title: Cellular prion: a novel regulator of decidual cell function at the maternal-fetal interface.
Article Snippet: Cellular prion (PRNP) is a GPI-anchored extrinsic membrane glycoprotein, which has been implicated in mouse decidualization.. However, the molecular function of this protein in mouse decidua is not known.. In this article, we have characterized and elucidated the possible role of PRNP in mouse decidua.

Article Title: What Happens Inside the Germinating Grain After Microbial Decontamination by Pulsed Electric Field? Data-Driven Multi-Omics Helps Find the Answer
Article Snippet: Real-time primers and probes for the quantification of Fusarium species DNA are provided in .

Article Title: Selection and validation of reference genes for quantitative expression analysis of regeneration-related genes in Cheilomenes sexmaculata by real-time qRT-PCR.
Article Snippet: The regeneration of missing body parts is a fascinating phenomenon that has captured the interest of scientists since time immemorial.. Several organisms ranging from planaria and echinoderms to vertebrates have the potential to regenerate their distal limb structures or primordia that are lost due to an injury or any experimental manipulation [1, 2].. Recent studies in ladybird beetles (Coleoptera: Coccinellidae) have shown high incidences of regeneration [3, 4].

Article Title: Evaluation of physical, chemical, and microbiological characteristics of waste stabilization ponds, Giza, Egypt
Article Snippet: The outlet parameters of the Atfih WWTP met the Egyptian legislation (the Egyptian Code 501/2015, category D) for the irrigation of trees (Table 2). qPCR specificity, sensitivity, and detection limits Each set of real-time primers and probes utilized in this investigation was assessed for specificity.

Transfection:

Article Title: miR-146a is Critical for Orchestrating Mycobacterium fortuitum Survival Through Anti-inflammatory and M2 Macrophage Responses in Fish.
Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Article Title: Combined analysis of mRNA and miRNA reveals the mechanism of pacific white shrimp ( Litopenaeus vannamei ) under acute alkalinity stress
Article Snippet: The total RNA used for the previous RNA-Seq and small RNA analysis was reverse transcribed into cDNA using a mixture of Stem loop primers and real-time primers respectively. qRT-PCR cycles were as follows: 30 s at 95°C, followed by 40 cycles of 5 s at 95°C and 30 s at 60°C. β -actin was used as internal controls to normalize the experimental data of the miRNAs and mRNAs.

Article Title: Application of Pulsed Electric Field During Malting: Impact on Fusarium Species Growth and Mycotoxin Production.
Article Snippet: Real-time primers and probes for the quantification of Fusarium species DNA are provided in Table 5.

Article Title: Knockdown of orthotospovirus-derived silencing suppressor gene by plant-mediated RNAi approach induces viral resistance in tomato
Article Snippet: RNA interference (RNAi) shows significant effectiveness in conferring resistance to viral infections in various plants.. The current study was conducted to develop transgenic tomato (Solanum lycopersicum cv.. Rio Grande) lines expressing a 320 bp conserved inverted region of the integral 35S promoter region.

Article Title: Cellular prion: a novel regulator of decidual cell function at the maternal-fetal interface.
Article Snippet: Cellular prion (PRNP) is a GPI-anchored extrinsic membrane glycoprotein, which has been implicated in mouse decidualization.. However, the molecular function of this protein in mouse decidua is not known.. In this article, we have characterized and elucidated the possible role of PRNP in mouse decidua.

Article Title: What Happens Inside the Germinating Grain After Microbial Decontamination by Pulsed Electric Field? Data-Driven Multi-Omics Helps Find the Answer
Article Snippet: Real-time primers and probes for the quantification of Fusarium species DNA are provided in .

Article Title: Selection and validation of reference genes for quantitative expression analysis of regeneration-related genes in Cheilomenes sexmaculata by real-time qRT-PCR.
Article Snippet: The regeneration of missing body parts is a fascinating phenomenon that has captured the interest of scientists since time immemorial.. Several organisms ranging from planaria and echinoderms to vertebrates have the potential to regenerate their distal limb structures or primordia that are lost due to an injury or any experimental manipulation [1, 2].. Recent studies in ladybird beetles (Coleoptera: Coccinellidae) have shown high incidences of regeneration [3, 4].

Article Title: Evaluation of physical, chemical, and microbiological characteristics of waste stabilization ponds, Giza, Egypt
Article Snippet: The outlet parameters of the Atfih WWTP met the Egyptian legislation (the Egyptian Code 501/2015, category D) for the irrigation of trees (Table 2). qPCR specificity, sensitivity, and detection limits Each set of real-time primers and probes utilized in this investigation was assessed for specificity.

Isolation:

Article Title: miR-146a is Critical for Orchestrating Mycobacterium fortuitum Survival Through Anti-inflammatory and M2 Macrophage Responses in Fish.
Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Article Title: Combined analysis of mRNA and miRNA reveals the mechanism of pacific white shrimp ( Litopenaeus vannamei ) under acute alkalinity stress
Article Snippet: The total RNA used for the previous RNA-Seq and small RNA analysis was reverse transcribed into cDNA using a mixture of Stem loop primers and real-time primers respectively. qRT-PCR cycles were as follows: 30 s at 95°C, followed by 40 cycles of 5 s at 95°C and 30 s at 60°C. β -actin was used as internal controls to normalize the experimental data of the miRNAs and mRNAs.

Article Title: Application of Pulsed Electric Field During Malting: Impact on Fusarium Species Growth and Mycotoxin Production.
Article Snippet: Real-time primers and probes for the quantification of Fusarium species DNA are provided in Table 5.

Article Title: Knockdown of orthotospovirus-derived silencing suppressor gene by plant-mediated RNAi approach induces viral resistance in tomato
Article Snippet: RNA interference (RNAi) shows significant effectiveness in conferring resistance to viral infections in various plants.. The current study was conducted to develop transgenic tomato (Solanum lycopersicum cv.. Rio Grande) lines expressing a 320 bp conserved inverted region of the integral 35S promoter region.

Article Title: Cellular prion: a novel regulator of decidual cell function at the maternal-fetal interface.
Article Snippet: Cellular prion (PRNP) is a GPI-anchored extrinsic membrane glycoprotein, which has been implicated in mouse decidualization.. However, the molecular function of this protein in mouse decidua is not known.. In this article, we have characterized and elucidated the possible role of PRNP in mouse decidua.

Article Title: What Happens Inside the Germinating Grain After Microbial Decontamination by Pulsed Electric Field? Data-Driven Multi-Omics Helps Find the Answer
Article Snippet: Real-time primers and probes for the quantification of Fusarium species DNA are provided in .

Article Title: Selection and validation of reference genes for quantitative expression analysis of regeneration-related genes in Cheilomenes sexmaculata by real-time qRT-PCR.
Article Snippet: The regeneration of missing body parts is a fascinating phenomenon that has captured the interest of scientists since time immemorial.. Several organisms ranging from planaria and echinoderms to vertebrates have the potential to regenerate their distal limb structures or primordia that are lost due to an injury or any experimental manipulation [1, 2].. Recent studies in ladybird beetles (Coleoptera: Coccinellidae) have shown high incidences of regeneration [3, 4].

Article Title: Evaluation of physical, chemical, and microbiological characteristics of waste stabilization ponds, Giza, Egypt
Article Snippet: The outlet parameters of the Atfih WWTP met the Egyptian legislation (the Egyptian Code 501/2015, category D) for the irrigation of trees (Table 2). qPCR specificity, sensitivity, and detection limits Each set of real-time primers and probes utilized in this investigation was assessed for specificity.

MTT Assay:

Article Title: miR-146a is Critical for Orchestrating Mycobacterium fortuitum Survival Through Anti-inflammatory and M2 Macrophage Responses in Fish.
Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Article Title: Combined analysis of mRNA and miRNA reveals the mechanism of pacific white shrimp ( Litopenaeus vannamei ) under acute alkalinity stress
Article Snippet: The total RNA used for the previous RNA-Seq and small RNA analysis was reverse transcribed into cDNA using a mixture of Stem loop primers and real-time primers respectively. qRT-PCR cycles were as follows: 30 s at 95°C, followed by 40 cycles of 5 s at 95°C and 30 s at 60°C. β -actin was used as internal controls to normalize the experimental data of the miRNAs and mRNAs.

Article Title: Application of Pulsed Electric Field During Malting: Impact on Fusarium Species Growth and Mycotoxin Production.
Article Snippet: Real-time primers and probes for the quantification of Fusarium species DNA are provided in Table 5.

Article Title: Knockdown of orthotospovirus-derived silencing suppressor gene by plant-mediated RNAi approach induces viral resistance in tomato
Article Snippet: RNA interference (RNAi) shows significant effectiveness in conferring resistance to viral infections in various plants.. The current study was conducted to develop transgenic tomato (Solanum lycopersicum cv.. Rio Grande) lines expressing a 320 bp conserved inverted region of the integral 35S promoter region.

Article Title: Cellular prion: a novel regulator of decidual cell function at the maternal-fetal interface.
Article Snippet: Cellular prion (PRNP) is a GPI-anchored extrinsic membrane glycoprotein, which has been implicated in mouse decidualization.. However, the molecular function of this protein in mouse decidua is not known.. In this article, we have characterized and elucidated the possible role of PRNP in mouse decidua.

Article Title: What Happens Inside the Germinating Grain After Microbial Decontamination by Pulsed Electric Field? Data-Driven Multi-Omics Helps Find the Answer
Article Snippet: Real-time primers and probes for the quantification of Fusarium species DNA are provided in .

Article Title: Selection and validation of reference genes for quantitative expression analysis of regeneration-related genes in Cheilomenes sexmaculata by real-time qRT-PCR.
Article Snippet: The regeneration of missing body parts is a fascinating phenomenon that has captured the interest of scientists since time immemorial.. Several organisms ranging from planaria and echinoderms to vertebrates have the potential to regenerate their distal limb structures or primordia that are lost due to an injury or any experimental manipulation [1, 2].. Recent studies in ladybird beetles (Coleoptera: Coccinellidae) have shown high incidences of regeneration [3, 4].

Article Title: Evaluation of physical, chemical, and microbiological characteristics of waste stabilization ponds, Giza, Egypt
Article Snippet: The outlet parameters of the Atfih WWTP met the Egyptian legislation (the Egyptian Code 501/2015, category D) for the irrigation of trees (Table 2). qPCR specificity, sensitivity, and detection limits Each set of real-time primers and probes utilized in this investigation was assessed for specificity.

Incubation:

Article Title: miR-146a is Critical for Orchestrating Mycobacterium fortuitum Survival Through Anti-inflammatory and M2 Macrophage Responses in Fish.
Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Article Title: Combined analysis of mRNA and miRNA reveals the mechanism of pacific white shrimp ( Litopenaeus vannamei ) under acute alkalinity stress
Article Snippet: The total RNA used for the previous RNA-Seq and small RNA analysis was reverse transcribed into cDNA using a mixture of Stem loop primers and real-time primers respectively. qRT-PCR cycles were as follows: 30 s at 95°C, followed by 40 cycles of 5 s at 95°C and 30 s at 60°C. β -actin was used as internal controls to normalize the experimental data of the miRNAs and mRNAs.

Article Title: Application of Pulsed Electric Field During Malting: Impact on Fusarium Species Growth and Mycotoxin Production.
Article Snippet: Real-time primers and probes for the quantification of Fusarium species DNA are provided in Table 5.

Article Title: Knockdown of orthotospovirus-derived silencing suppressor gene by plant-mediated RNAi approach induces viral resistance in tomato
Article Snippet: RNA interference (RNAi) shows significant effectiveness in conferring resistance to viral infections in various plants.. The current study was conducted to develop transgenic tomato (Solanum lycopersicum cv.. Rio Grande) lines expressing a 320 bp conserved inverted region of the integral 35S promoter region.

Article Title: Cellular prion: a novel regulator of decidual cell function at the maternal-fetal interface.
Article Snippet: Cellular prion (PRNP) is a GPI-anchored extrinsic membrane glycoprotein, which has been implicated in mouse decidualization.. However, the molecular function of this protein in mouse decidua is not known.. In this article, we have characterized and elucidated the possible role of PRNP in mouse decidua.

Article Title: What Happens Inside the Germinating Grain After Microbial Decontamination by Pulsed Electric Field? Data-Driven Multi-Omics Helps Find the Answer
Article Snippet: Real-time primers and probes for the quantification of Fusarium species DNA are provided in .

Article Title: Selection and validation of reference genes for quantitative expression analysis of regeneration-related genes in Cheilomenes sexmaculata by real-time qRT-PCR.
Article Snippet: The regeneration of missing body parts is a fascinating phenomenon that has captured the interest of scientists since time immemorial.. Several organisms ranging from planaria and echinoderms to vertebrates have the potential to regenerate their distal limb structures or primordia that are lost due to an injury or any experimental manipulation [1, 2].. Recent studies in ladybird beetles (Coleoptera: Coccinellidae) have shown high incidences of regeneration [3, 4].

Article Title: Evaluation of physical, chemical, and microbiological characteristics of waste stabilization ponds, Giza, Egypt
Article Snippet: The outlet parameters of the Atfih WWTP met the Egyptian legislation (the Egyptian Code 501/2015, category D) for the irrigation of trees (Table 2). qPCR specificity, sensitivity, and detection limits Each set of real-time primers and probes utilized in this investigation was assessed for specificity.

In Silico:

Article Title: miR-146a is Critical for Orchestrating Mycobacterium fortuitum Survival Through Anti-inflammatory and M2 Macrophage Responses in Fish.
Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Article Title: Combined analysis of mRNA and miRNA reveals the mechanism of pacific white shrimp ( Litopenaeus vannamei ) under acute alkalinity stress
Article Snippet: The total RNA used for the previous RNA-Seq and small RNA analysis was reverse transcribed into cDNA using a mixture of Stem loop primers and real-time primers respectively. qRT-PCR cycles were as follows: 30 s at 95°C, followed by 40 cycles of 5 s at 95°C and 30 s at 60°C. β -actin was used as internal controls to normalize the experimental data of the miRNAs and mRNAs.

Article Title: Application of Pulsed Electric Field During Malting: Impact on Fusarium Species Growth and Mycotoxin Production.
Article Snippet: Real-time primers and probes for the quantification of Fusarium species DNA are provided in Table 5.

Article Title: Knockdown of orthotospovirus-derived silencing suppressor gene by plant-mediated RNAi approach induces viral resistance in tomato
Article Snippet: RNA interference (RNAi) shows significant effectiveness in conferring resistance to viral infections in various plants.. The current study was conducted to develop transgenic tomato (Solanum lycopersicum cv.. Rio Grande) lines expressing a 320 bp conserved inverted region of the integral 35S promoter region.

Article Title: Cellular prion: a novel regulator of decidual cell function at the maternal-fetal interface.
Article Snippet: Cellular prion (PRNP) is a GPI-anchored extrinsic membrane glycoprotein, which has been implicated in mouse decidualization.. However, the molecular function of this protein in mouse decidua is not known.. In this article, we have characterized and elucidated the possible role of PRNP in mouse decidua.

Article Title: What Happens Inside the Germinating Grain After Microbial Decontamination by Pulsed Electric Field? Data-Driven Multi-Omics Helps Find the Answer
Article Snippet: Real-time primers and probes for the quantification of Fusarium species DNA are provided in .

Article Title: Selection and validation of reference genes for quantitative expression analysis of regeneration-related genes in Cheilomenes sexmaculata by real-time qRT-PCR.
Article Snippet: The regeneration of missing body parts is a fascinating phenomenon that has captured the interest of scientists since time immemorial.. Several organisms ranging from planaria and echinoderms to vertebrates have the potential to regenerate their distal limb structures or primordia that are lost due to an injury or any experimental manipulation [1, 2].. Recent studies in ladybird beetles (Coleoptera: Coccinellidae) have shown high incidences of regeneration [3, 4].

Article Title: Evaluation of physical, chemical, and microbiological characteristics of waste stabilization ponds, Giza, Egypt
Article Snippet: The outlet parameters of the Atfih WWTP met the Egyptian legislation (the Egyptian Code 501/2015, category D) for the irrigation of trees (Table 2). qPCR specificity, sensitivity, and detection limits Each set of real-time primers and probes utilized in this investigation was assessed for specificity.

Tandem Mass Spectroscopy:

Article Title: miR-146a is Critical for Orchestrating Mycobacterium fortuitum Survival Through Anti-inflammatory and M2 Macrophage Responses in Fish.
Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Article Title: Combined analysis of mRNA and miRNA reveals the mechanism of pacific white shrimp ( Litopenaeus vannamei ) under acute alkalinity stress
Article Snippet: The total RNA used for the previous RNA-Seq and small RNA analysis was reverse transcribed into cDNA using a mixture of Stem loop primers and real-time primers respectively. qRT-PCR cycles were as follows: 30 s at 95°C, followed by 40 cycles of 5 s at 95°C and 30 s at 60°C. β -actin was used as internal controls to normalize the experimental data of the miRNAs and mRNAs.

Article Title: Application of Pulsed Electric Field During Malting: Impact on Fusarium Species Growth and Mycotoxin Production.
Article Snippet: Real-time primers and probes for the quantification of Fusarium species DNA are provided in Table 5.

Article Title: Knockdown of orthotospovirus-derived silencing suppressor gene by plant-mediated RNAi approach induces viral resistance in tomato
Article Snippet: RNA interference (RNAi) shows significant effectiveness in conferring resistance to viral infections in various plants.. The current study was conducted to develop transgenic tomato (Solanum lycopersicum cv.. Rio Grande) lines expressing a 320 bp conserved inverted region of the integral 35S promoter region.

Article Title: Cellular prion: a novel regulator of decidual cell function at the maternal-fetal interface.
Article Snippet: Cellular prion (PRNP) is a GPI-anchored extrinsic membrane glycoprotein, which has been implicated in mouse decidualization.. However, the molecular function of this protein in mouse decidua is not known.. In this article, we have characterized and elucidated the possible role of PRNP in mouse decidua.

Article Title: What Happens Inside the Germinating Grain After Microbial Decontamination by Pulsed Electric Field? Data-Driven Multi-Omics Helps Find the Answer
Article Snippet: Real-time primers and probes for the quantification of Fusarium species DNA are provided in .

Article Title: Selection and validation of reference genes for quantitative expression analysis of regeneration-related genes in Cheilomenes sexmaculata by real-time qRT-PCR.
Article Snippet: The regeneration of missing body parts is a fascinating phenomenon that has captured the interest of scientists since time immemorial.. Several organisms ranging from planaria and echinoderms to vertebrates have the potential to regenerate their distal limb structures or primordia that are lost due to an injury or any experimental manipulation [1, 2].. Recent studies in ladybird beetles (Coleoptera: Coccinellidae) have shown high incidences of regeneration [3, 4].

Article Title: Evaluation of physical, chemical, and microbiological characteristics of waste stabilization ponds, Giza, Egypt
Article Snippet: The outlet parameters of the Atfih WWTP met the Egyptian legislation (the Egyptian Code 501/2015, category D) for the irrigation of trees (Table 2). qPCR specificity, sensitivity, and detection limits Each set of real-time primers and probes utilized in this investigation was assessed for specificity.

Filtration:

Article Title: miR-146a is Critical for Orchestrating Mycobacterium fortuitum Survival Through Anti-inflammatory and M2 Macrophage Responses in Fish.
Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Article Title: Combined analysis of mRNA and miRNA reveals the mechanism of pacific white shrimp ( Litopenaeus vannamei ) under acute alkalinity stress
Article Snippet: The total RNA used for the previous RNA-Seq and small RNA analysis was reverse transcribed into cDNA using a mixture of Stem loop primers and real-time primers respectively. qRT-PCR cycles were as follows: 30 s at 95°C, followed by 40 cycles of 5 s at 95°C and 30 s at 60°C. β -actin was used as internal controls to normalize the experimental data of the miRNAs and mRNAs.

Article Title: Application of Pulsed Electric Field During Malting: Impact on Fusarium Species Growth and Mycotoxin Production.
Article Snippet: Real-time primers and probes for the quantification of Fusarium species DNA are provided in Table 5.

Article Title: Knockdown of orthotospovirus-derived silencing suppressor gene by plant-mediated RNAi approach induces viral resistance in tomato
Article Snippet: RNA interference (RNAi) shows significant effectiveness in conferring resistance to viral infections in various plants.. The current study was conducted to develop transgenic tomato (Solanum lycopersicum cv.. Rio Grande) lines expressing a 320 bp conserved inverted region of the integral 35S promoter region.

Article Title: Cellular prion: a novel regulator of decidual cell function at the maternal-fetal interface.
Article Snippet: Cellular prion (PRNP) is a GPI-anchored extrinsic membrane glycoprotein, which has been implicated in mouse decidualization.. However, the molecular function of this protein in mouse decidua is not known.. In this article, we have characterized and elucidated the possible role of PRNP in mouse decidua.

Article Title: What Happens Inside the Germinating Grain After Microbial Decontamination by Pulsed Electric Field? Data-Driven Multi-Omics Helps Find the Answer
Article Snippet: Real-time primers and probes for the quantification of Fusarium species DNA are provided in .

Article Title: Selection and validation of reference genes for quantitative expression analysis of regeneration-related genes in Cheilomenes sexmaculata by real-time qRT-PCR.
Article Snippet: The regeneration of missing body parts is a fascinating phenomenon that has captured the interest of scientists since time immemorial.. Several organisms ranging from planaria and echinoderms to vertebrates have the potential to regenerate their distal limb structures or primordia that are lost due to an injury or any experimental manipulation [1, 2].. Recent studies in ladybird beetles (Coleoptera: Coccinellidae) have shown high incidences of regeneration [3, 4].

Article Title: Evaluation of physical, chemical, and microbiological characteristics of waste stabilization ponds, Giza, Egypt
Article Snippet: The outlet parameters of the Atfih WWTP met the Egyptian legislation (the Egyptian Code 501/2015, category D) for the irrigation of trees (Table 2). qPCR specificity, sensitivity, and detection limits Each set of real-time primers and probes utilized in this investigation was assessed for specificity.

Control:

Article Title: miR-146a is Critical for Orchestrating Mycobacterium fortuitum Survival Through Anti-inflammatory and M2 Macrophage Responses in Fish.
Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Article Title: Combined analysis of mRNA and miRNA reveals the mechanism of pacific white shrimp ( Litopenaeus vannamei ) under acute alkalinity stress
Article Snippet: The total RNA used for the previous RNA-Seq and small RNA analysis was reverse transcribed into cDNA using a mixture of Stem loop primers and real-time primers respectively. qRT-PCR cycles were as follows: 30 s at 95°C, followed by 40 cycles of 5 s at 95°C and 30 s at 60°C. β -actin was used as internal controls to normalize the experimental data of the miRNAs and mRNAs.

Article Title: Application of Pulsed Electric Field During Malting: Impact on Fusarium Species Growth and Mycotoxin Production.
Article Snippet: Real-time primers and probes for the quantification of Fusarium species DNA are provided in Table 5.

Article Title: Knockdown of orthotospovirus-derived silencing suppressor gene by plant-mediated RNAi approach induces viral resistance in tomato
Article Snippet: RNA interference (RNAi) shows significant effectiveness in conferring resistance to viral infections in various plants.. The current study was conducted to develop transgenic tomato (Solanum lycopersicum cv.. Rio Grande) lines expressing a 320 bp conserved inverted region of the integral 35S promoter region.

Article Title: Cellular prion: a novel regulator of decidual cell function at the maternal-fetal interface.
Article Snippet: Cellular prion (PRNP) is a GPI-anchored extrinsic membrane glycoprotein, which has been implicated in mouse decidualization.. However, the molecular function of this protein in mouse decidua is not known.. In this article, we have characterized and elucidated the possible role of PRNP in mouse decidua.

Article Title: What Happens Inside the Germinating Grain After Microbial Decontamination by Pulsed Electric Field? Data-Driven Multi-Omics Helps Find the Answer
Article Snippet: Real-time primers and probes for the quantification of Fusarium species DNA are provided in .

Article Title: Selection and validation of reference genes for quantitative expression analysis of regeneration-related genes in Cheilomenes sexmaculata by real-time qRT-PCR.
Article Snippet: The regeneration of missing body parts is a fascinating phenomenon that has captured the interest of scientists since time immemorial.. Several organisms ranging from planaria and echinoderms to vertebrates have the potential to regenerate their distal limb structures or primordia that are lost due to an injury or any experimental manipulation [1, 2].. Recent studies in ladybird beetles (Coleoptera: Coccinellidae) have shown high incidences of regeneration [3, 4].

Article Title: Evaluation of physical, chemical, and microbiological characteristics of waste stabilization ponds, Giza, Egypt
Article Snippet: The outlet parameters of the Atfih WWTP met the Egyptian legislation (the Egyptian Code 501/2015, category D) for the irrigation of trees (Table 2). qPCR specificity, sensitivity, and detection limits Each set of real-time primers and probes utilized in this investigation was assessed for specificity.

Sequencing:

Article Title: miR-146a is Critical for Orchestrating Mycobacterium fortuitum Survival Through Anti-inflammatory and M2 Macrophage Responses in Fish.
Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Article Title: Combined analysis of mRNA and miRNA reveals the mechanism of pacific white shrimp ( Litopenaeus vannamei ) under acute alkalinity stress
Article Snippet: The total RNA used for the previous RNA-Seq and small RNA analysis was reverse transcribed into cDNA using a mixture of Stem loop primers and real-time primers respectively. qRT-PCR cycles were as follows: 30 s at 95°C, followed by 40 cycles of 5 s at 95°C and 30 s at 60°C. β -actin was used as internal controls to normalize the experimental data of the miRNAs and mRNAs.

Article Title: Application of Pulsed Electric Field During Malting: Impact on Fusarium Species Growth and Mycotoxin Production.
Article Snippet: Real-time primers and probes for the quantification of Fusarium species DNA are provided in Table 5.

Article Title: Knockdown of orthotospovirus-derived silencing suppressor gene by plant-mediated RNAi approach induces viral resistance in tomato
Article Snippet: RNA interference (RNAi) shows significant effectiveness in conferring resistance to viral infections in various plants.. The current study was conducted to develop transgenic tomato (Solanum lycopersicum cv.. Rio Grande) lines expressing a 320 bp conserved inverted region of the integral 35S promoter region.

Article Title: Cellular prion: a novel regulator of decidual cell function at the maternal-fetal interface.
Article Snippet: Cellular prion (PRNP) is a GPI-anchored extrinsic membrane glycoprotein, which has been implicated in mouse decidualization.. However, the molecular function of this protein in mouse decidua is not known.. In this article, we have characterized and elucidated the possible role of PRNP in mouse decidua.

Article Title: What Happens Inside the Germinating Grain After Microbial Decontamination by Pulsed Electric Field? Data-Driven Multi-Omics Helps Find the Answer
Article Snippet: Real-time primers and probes for the quantification of Fusarium species DNA are provided in .

Article Title: Selection and validation of reference genes for quantitative expression analysis of regeneration-related genes in Cheilomenes sexmaculata by real-time qRT-PCR.
Article Snippet: The regeneration of missing body parts is a fascinating phenomenon that has captured the interest of scientists since time immemorial.. Several organisms ranging from planaria and echinoderms to vertebrates have the potential to regenerate their distal limb structures or primordia that are lost due to an injury or any experimental manipulation [1, 2].. Recent studies in ladybird beetles (Coleoptera: Coccinellidae) have shown high incidences of regeneration [3, 4].

Article Title: Evaluation of physical, chemical, and microbiological characteristics of waste stabilization ponds, Giza, Egypt
Article Snippet: The outlet parameters of the Atfih WWTP met the Egyptian legislation (the Egyptian Code 501/2015, category D) for the irrigation of trees (Table 2). qPCR specificity, sensitivity, and detection limits Each set of real-time primers and probes utilized in this investigation was assessed for specificity.

Derivative Assay:

Article Title: miR-146a is Critical for Orchestrating Mycobacterium fortuitum Survival Through Anti-inflammatory and M2 Macrophage Responses in Fish.
Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Article Title: Combined analysis of mRNA and miRNA reveals the mechanism of pacific white shrimp ( Litopenaeus vannamei ) under acute alkalinity stress
Article Snippet: The total RNA used for the previous RNA-Seq and small RNA analysis was reverse transcribed into cDNA using a mixture of Stem loop primers and real-time primers respectively. qRT-PCR cycles were as follows: 30 s at 95°C, followed by 40 cycles of 5 s at 95°C and 30 s at 60°C. β -actin was used as internal controls to normalize the experimental data of the miRNAs and mRNAs.

Article Title: Application of Pulsed Electric Field During Malting: Impact on Fusarium Species Growth and Mycotoxin Production.
Article Snippet: Real-time primers and probes for the quantification of Fusarium species DNA are provided in Table 5.

Article Title: Knockdown of orthotospovirus-derived silencing suppressor gene by plant-mediated RNAi approach induces viral resistance in tomato
Article Snippet: RNA interference (RNAi) shows significant effectiveness in conferring resistance to viral infections in various plants.. The current study was conducted to develop transgenic tomato (Solanum lycopersicum cv.. Rio Grande) lines expressing a 320 bp conserved inverted region of the integral 35S promoter region.

Article Title: Cellular prion: a novel regulator of decidual cell function at the maternal-fetal interface.
Article Snippet: Cellular prion (PRNP) is a GPI-anchored extrinsic membrane glycoprotein, which has been implicated in mouse decidualization.. However, the molecular function of this protein in mouse decidua is not known.. In this article, we have characterized and elucidated the possible role of PRNP in mouse decidua.

Article Title: What Happens Inside the Germinating Grain After Microbial Decontamination by Pulsed Electric Field? Data-Driven Multi-Omics Helps Find the Answer
Article Snippet: Real-time primers and probes for the quantification of Fusarium species DNA are provided in .

Article Title: Selection and validation of reference genes for quantitative expression analysis of regeneration-related genes in Cheilomenes sexmaculata by real-time qRT-PCR.
Article Snippet: The regeneration of missing body parts is a fascinating phenomenon that has captured the interest of scientists since time immemorial.. Several organisms ranging from planaria and echinoderms to vertebrates have the potential to regenerate their distal limb structures or primordia that are lost due to an injury or any experimental manipulation [1, 2].. Recent studies in ladybird beetles (Coleoptera: Coccinellidae) have shown high incidences of regeneration [3, 4].

Article Title: Evaluation of physical, chemical, and microbiological characteristics of waste stabilization ponds, Giza, Egypt
Article Snippet: The outlet parameters of the Atfih WWTP met the Egyptian legislation (the Egyptian Code 501/2015, category D) for the irrigation of trees (Table 2). qPCR specificity, sensitivity, and detection limits Each set of real-time primers and probes utilized in this investigation was assessed for specificity.

Synthesized:

Article Title: miR-146a is Critical for Orchestrating Mycobacterium fortuitum Survival Through Anti-inflammatory and M2 Macrophage Responses in Fish.
Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Article Title: Combined analysis of mRNA and miRNA reveals the mechanism of pacific white shrimp ( Litopenaeus vannamei ) under acute alkalinity stress
Article Snippet: The total RNA used for the previous RNA-Seq and small RNA analysis was reverse transcribed into cDNA using a mixture of Stem loop primers and real-time primers respectively. qRT-PCR cycles were as follows: 30 s at 95°C, followed by 40 cycles of 5 s at 95°C and 30 s at 60°C. β -actin was used as internal controls to normalize the experimental data of the miRNAs and mRNAs.

Article Title: Application of Pulsed Electric Field During Malting: Impact on Fusarium Species Growth and Mycotoxin Production.
Article Snippet: Real-time primers and probes for the quantification of Fusarium species DNA are provided in Table 5.

Article Title: Knockdown of orthotospovirus-derived silencing suppressor gene by plant-mediated RNAi approach induces viral resistance in tomato
Article Snippet: RNA interference (RNAi) shows significant effectiveness in conferring resistance to viral infections in various plants.. The current study was conducted to develop transgenic tomato (Solanum lycopersicum cv.. Rio Grande) lines expressing a 320 bp conserved inverted region of the integral 35S promoter region.

Article Title: Cellular prion: a novel regulator of decidual cell function at the maternal-fetal interface.
Article Snippet: Cellular prion (PRNP) is a GPI-anchored extrinsic membrane glycoprotein, which has been implicated in mouse decidualization.. However, the molecular function of this protein in mouse decidua is not known.. In this article, we have characterized and elucidated the possible role of PRNP in mouse decidua.

Article Title: What Happens Inside the Germinating Grain After Microbial Decontamination by Pulsed Electric Field? Data-Driven Multi-Omics Helps Find the Answer
Article Snippet: Real-time primers and probes for the quantification of Fusarium species DNA are provided in .

Article Title: Selection and validation of reference genes for quantitative expression analysis of regeneration-related genes in Cheilomenes sexmaculata by real-time qRT-PCR.
Article Snippet: The regeneration of missing body parts is a fascinating phenomenon that has captured the interest of scientists since time immemorial.. Several organisms ranging from planaria and echinoderms to vertebrates have the potential to regenerate their distal limb structures or primordia that are lost due to an injury or any experimental manipulation [1, 2].. Recent studies in ladybird beetles (Coleoptera: Coccinellidae) have shown high incidences of regeneration [3, 4].

Article Title: Evaluation of physical, chemical, and microbiological characteristics of waste stabilization ponds, Giza, Egypt
Article Snippet: The outlet parameters of the Atfih WWTP met the Egyptian legislation (the Egyptian Code 501/2015, category D) for the irrigation of trees (Table 2). qPCR specificity, sensitivity, and detection limits Each set of real-time primers and probes utilized in this investigation was assessed for specificity.

Quantitative RT-PCR:

Article Title: miR-146a is Critical for Orchestrating Mycobacterium fortuitum Survival Through Anti-inflammatory and M2 Macrophage Responses in Fish.
Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Article Title: Combined analysis of mRNA and miRNA reveals the mechanism of pacific white shrimp ( Litopenaeus vannamei ) under acute alkalinity stress
Article Snippet: The total RNA used for the previous RNA-Seq and small RNA analysis was reverse transcribed into cDNA using a mixture of Stem loop primers and real-time primers respectively. qRT-PCR cycles were as follows: 30 s at 95°C, followed by 40 cycles of 5 s at 95°C and 30 s at 60°C. β -actin was used as internal controls to normalize the experimental data of the miRNAs and mRNAs.

Article Title: Application of Pulsed Electric Field During Malting: Impact on Fusarium Species Growth and Mycotoxin Production.
Article Snippet: Real-time primers and probes for the quantification of Fusarium species DNA are provided in Table 5.

Article Title: Knockdown of orthotospovirus-derived silencing suppressor gene by plant-mediated RNAi approach induces viral resistance in tomato
Article Snippet: RNA interference (RNAi) shows significant effectiveness in conferring resistance to viral infections in various plants.. The current study was conducted to develop transgenic tomato (Solanum lycopersicum cv.. Rio Grande) lines expressing a 320 bp conserved inverted region of the integral 35S promoter region.

Article Title: Cellular prion: a novel regulator of decidual cell function at the maternal-fetal interface.
Article Snippet: Cellular prion (PRNP) is a GPI-anchored extrinsic membrane glycoprotein, which has been implicated in mouse decidualization.. However, the molecular function of this protein in mouse decidua is not known.. In this article, we have characterized and elucidated the possible role of PRNP in mouse decidua.

Article Title: What Happens Inside the Germinating Grain After Microbial Decontamination by Pulsed Electric Field? Data-Driven Multi-Omics Helps Find the Answer
Article Snippet: Real-time primers and probes for the quantification of Fusarium species DNA are provided in .

Article Title: Selection and validation of reference genes for quantitative expression analysis of regeneration-related genes in Cheilomenes sexmaculata by real-time qRT-PCR.
Article Snippet: The regeneration of missing body parts is a fascinating phenomenon that has captured the interest of scientists since time immemorial.. Several organisms ranging from planaria and echinoderms to vertebrates have the potential to regenerate their distal limb structures or primordia that are lost due to an injury or any experimental manipulation [1, 2].. Recent studies in ladybird beetles (Coleoptera: Coccinellidae) have shown high incidences of regeneration [3, 4].

Article Title: Evaluation of physical, chemical, and microbiological characteristics of waste stabilization ponds, Giza, Egypt
Article Snippet: The outlet parameters of the Atfih WWTP met the Egyptian legislation (the Egyptian Code 501/2015, category D) for the irrigation of trees (Table 2). qPCR specificity, sensitivity, and detection limits Each set of real-time primers and probes utilized in this investigation was assessed for specificity.



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